This article is part of a special issue entitled: photosynthesis research for sustainability: keys to produce clean energy. The absence of the ligand for chlorophyll 612 leads to the loss of one Chl a and of lutein, again as in vitro, indicating the feasibility of the method. It is shown that CP24-WT coordinates 10 chlorophylls and 2 carotenoid molecules and has properties identical to those of the reconstituted complex, demonstrating that the complex self-assembled in vitro assumes the same folding as in the plant. The presence of the His-tag allowed the purification of CP24-WT and of CP24-612 mutant in their native states. Both complexes efficiently integrated into the thylakoid membrane and assembled into the PSII supercomplexes, indicating that the His-tag does not impair the organization in vivo. We have complemented a CP24 knock-out mutant of Arabidopsis thaliana with the CP24 (Lhcb6) gene carrying a His-tag and with a mutated version lacking the ligand for chlorophyll 612, a specific pigment that in vitro experiments have indicated as the lowest energy site of the complex.
In this work, we have envisaged a way to bridge the gap between in vitro and in vivo studies by knocking out in vivo pigments that have been proposed to play an important role in excitation energy transfer between the complexes or in photoprotection. In plants, however, these proteins are associated with multiple complexes of the photosystems and function within this framework. Combining spectroscopy, modeling and mutation analyses, the role of individual pigments in these processes has been highlighted in vitro.
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SNS-HDR Pro 1.4.22.1 torrent download: Torrent created: 01:27:54: Torrent added. The calculations of the energy spectra of protons carried out with this model are in good agreement with experiment. All functions entering into the matrix element are given analytically, which allows the analytic calculation of multidimensional integrals entering into the matrix element. The reaction matrix element is calculated on the basis of the t-matrix distorted-wave approximation.
We propose to calculate the amplitude for the two-stage processes in reactions of nucleon and cluster knock out from light nuclei by medium-energy protons as a coherent sum of amplitudes of the probability of the quasielastic pp (or px) interaction and of inelastic proton scattering by the target nucleus with formation of excited states of the intermediate nucleus and with their subsequent decay with emission of a nucleon or a particle x. Fifty-nine participants (37 males, 22 females), ages 16 to 60 years, were included in the review, all
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